Biosynthesis of Plasma Lipoproteins

Biosynthesis of plasma lipoproteins by rat liver ribosomes.

Rat liver ribosomes in uifro incorporated labeled amino acids into proteins which showed the immunological behavior of the plasma lipoproteins. The presence of carrier lipoprotein or carrier lipid was required for the isolation and ultracentrifugal purification of immunologically reactive, labeled low and high density lipoproteins. The identity of the lipoproteins was further characterized by s...

Evaluation of plasma lipids and lipoproteins in patients with psoriasis

Psoriasis is an epidermal disease characterized by chronic relapsing papillon squamous erythematous plaques which may develop in the skin of elbows, knees and extensor surfaces. The cause of psoriasis is unknown. The primary etiologic factor may be related to excessive uncontrolled synthesis is resulting from changes in (1) the function of growth factors ( EGF),(2) presence of proto- oncogenes,...

Isolation of plasma lipoproteins

Lipoproteins were isolated from plasma of man, dog, rabbit, rat, and chicken by ultracentrifugation in continuous density gradients using the B14 titanium and B15 titanium zonal rotors. Both the VLDL and the LDL of human plasma were separated easily from the HDL and from the other more plentiful plasma proteins by centrifugation for only 1 or 2 hr in the B14 or B15 rotor, respectively. Satisfac...

Biosynthesis of plasma lipoproteins. Incorporation of 14C-glucosamine by cells and subcellular fractions of rat liver.

The incorporation of glucosamlneJ4C into plasma lipoproteins has been measured in the intact rat, in liver slices, and in the liver microsome fraction. The labeling of lipoprotein was studied in microsome subfractions, both in viva with glucosammeJ4C and in vitro with UDP-N-acetylglucosamineW. After isolation of lipoproteins with the aid of carrier plasma, labeled lipoproteins were found in smo...

Plasma lipoproteins in fish.